Researchers have developed a way to grow human heart tissue that can be a model for advanced heart chambers, known as the atria. The tissue, which is derived from human-stimulated multi-cell cell cells (hiPCSs), bows, expresses genes and reacts to drugs in a similar way to a real human atrium. The model, which was described November 8 in the magazine Cell Phone Reports, be useful for evaluating disease and drug mechanisms for atrial fibrillation – the most common type of arrhythmia.
In contrast to a standard 2D culture, the cardiomyocytes derived from cells were cultured in a way that they form 3D tissue and heart tissue that is similar to the atrial heartbeat. Specifically, the cells showed expression of atrial genes, contractile power, restriction kinetics and relaxation, electrophysical properties, and pharmaceutical responses to selective atrial drugs. According to the authors, the heart tissue of engineering could be a model of human atrium for mechanical studies of atrial fibrillation and for pre-clinical drug screening.
"This is the first time of human atroxy tissue and human toxicity to be produced in vitro from a reliable source of hiPSCs," said the first author Marta Lemme of the Hamburg-Eppendorf University Medical Center. "This could be useful for academic laboratories and pharmaceutical industry, because of testing new potential drugs, we need to produce an in vitro model of atrial fibrillation. The first step to that is to get cells that are # 39; n similar to human atrial gerdiomiomyyocytes, "Lemme says.
The author of study Lemme and senior Thomas Eschenhagen of the University Medical Center, Hamburg-Eppendorf, have been able to achieve this aim by producing arthritis cardiomyytete of hiPSC using vitamin A metabolite called retinoic all-trans. This technique involves the re-programming of blood cells or skin taken by human donors to a condition similar to cellular cells and then treating these immature cells with a whole-trans-retinoic acid to & # 39 ; w convert into cardioxyytes similar to atrial.
"But the study of this study is the combination of the discrimination of hiPSC in atrial cardiomyocytes with a 3D environment," said Lemme. "In fact, we showed that the 3D environment favors the distinction towards atrial phenotype compared to a standard 2D culture. The specific value of our study is a direct comparison of 3D heart tissue & # 39 ; for engineering with indigenous human atrial tissue obtained by patients on molecular and functional levels. "
More than 33 million people worldwide suffer from atrial fibrillation, and numbers increase. Inconsistent high frequency restrictions in the atria increase the risk for blood clots, stroke, and heart failure. Unfortunately, existing treatments such as anti-pyrrhythmic drugs are affected and limited and can cause adverse effects. In addition, the development of new drugs has been prevented by the difficulty in devoting and maintaining human atrial cardiomyocytes, or muscle and heart cells. Animal models have limited projected power because they do not properly represent human cardiomyocytes physiology.
"These atrial muscle strips are a great opportunity to model atrial fibrillation in the meals and test drugs," said Lemme. "However, improvements can still be made to reach even higher likelihood with human atrial tissue. For us, the next step is to test different methods to stimulate arrhythmia, the remodeling of mechanisms of electric remodeling of atrial fibrillation and drug testing potential new. "
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